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THE INNOVATION 2010
Now including catcher particle detachment!
Cell separation from buffy coat or whole
blood in 30 minutes.
Fast & Easy!
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A new system for cell and protein
isolation |
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We develop and produce particularly user-friendly separation systems for cell- and protein research. Using our ready-to-go kits, researchers can separate multiple antigens from complex biological solutions in one single reaction. The technology platform developed by pluriSelect is based on the physical separation of differently sized particles using a set of innovative tools.
Our product series, pluriBead®, enables researchers to isolate cells, proteins, bacteria or viruses from whole blood, cord blood, cell culture and other heterogeneous biological liquids in a fast, simple and gentle procedure. |
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Advantages of the pluriBead®
cell and protein isolation technology |
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With
pluriBead®we offer a robust isolation technology for targets from different biological fluids. Throughout the development process we focused our attention on a simple but important rule: the ease of use. The design of our ready-to-use kits reflects this characteristic of our product: it is easily applicable in your daily laboratory routine. |
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Easy and
fast to use |
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No sample
pre-treatment necessary |
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Separation
at 4° to 37°C possible,
no cooling required |
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Simultaneous
separation of different cell types |
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Simultaneous
separation of cells and proteins |
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Variable
sample volume (300 µl to
15 ml) |
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Very low
cell stress |
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No extra
fix cost for your lab because
of ‘ready-to-go kit’
solution |
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Application
examples for your lab |
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Single cells in 30 minutes
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Isolate single cells from suspensions such as buffy coat, cell cultures or directly from whole blood. No additional purification of the sample material is necessary. The pluriBead catcher particles are directly mixed with the sample material and bind to the target cells. Employing a sieve-separation system the bound target cells are carefully separated from unbound material. Subsequently, the target cells are detached from the catcher particles under physiological conditions within 10 minutes. The target cells are now available as a single cells suspension and can be used for further experiments such as cell culture experiments, direct stimulation, or FACS analysis. For example, isolating CD3 cells from whole blood or buffy coat, you obtain single cells in 30 minutes. |
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RNA
Isolation
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RNA
isolation of specific cells: You can isolate cells from complex biological solutions (whole blood or cord blood) for PCR analysis in less than 10 minutes. |
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Cell
Activation |
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Activation
of a cell sub-population: You can isolate your target cells and stimulate them with various concentrations of cell activators or for different activation durations. Cell stimulation can be performed right after the cell isolation. Because of the fast and simple cell isolation, several experiments can be performed simultaneously. As a quick rinse of cells is sufficient and centrifugation is not required at any step of the procedure even non-adherent cells can be activated in this way using pluriBead®. |
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Simlutaneous
Isolation
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Simultaneous multi-target isolation. In case you handle only very small or particularly precious sample material, the pluriBead® separation technology is the gold standard for your isolation experiment. Sample volumes of just 300 µl are sufficient for multiple target isolation experiments. Again, sample preparation is not necessary! |
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Depletion
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Depletion:
You can remove a particular population of cells from your sample. Just employ the pluriBead® technology for a target cell or protein depletion (negative selection) and use the eluate for your experiment! |
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